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5' 5-Nitroindole

Return to Modified Bases Modifications

5-Nitroindole is currently the best universal base available. It does not favor any particular base-pairing (i.e., it does not support base-specific hydrogen bond formation), but does contribute to duplex stability through base-stacking interactions. Therefore, it is not as destabilizing to the duplex as mismatches between the standard bases. 5-Nitroindole directs random incorporation of any specific base when used as a template for DNA polymerase and partially blocks enzyme processivity.

Scientific Details
MW:340.2
Mod Code:/55NitInd/
Extinction Coefficient:16000
Absorbance Max:265 nm
Extinction Coefficient
(At Absorbance max):
17000
Pricing Details
ProductPricingOrder
100 nmole DNA Oligo $283.00 SGD Add To Order
250 nmole DNA Oligo $283.00 SGD Add To Order
1 umole DNA Oligo $427.00 SGD Add To Order
2 µmole DNA Oligo $500.00 SGD Add To Order
100 nmole RNA Oligo $283.00 SGD Add To Order
250 nmole RNA Oligo $283.00 SGD Add To Order
1 umole RNA Oligo $427.00 SGD Add To Order
Structure
Structure