Processing
Ultramer® DNA Oligonucleotides
Long, high-quality oligos for demanding applications such as cloning, ddRNAi, homology-directed repair, and gene construction
- Complete confidence in oligos that are verified by electrospray ionization-mass spectrometry*
Unrivalled control of oligo specifications withcustom formulation and mixing options
* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.
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Ultramer DNA Oligos
Formulated to 4 nmol or 20 nmol guaranteed yield. Shipped dry or resuspended to 100 µM in IDTE buffer, pH 8.0.
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Duplexed Ultramer DNA Oligos
2 oligos, annealed and delivered in a single tube. Formulated to 4 nmol or 20 nmol guaranteed yield. Shipped dry.
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Ultramer DNA Oligos
Shipped dry or resuspended to your specifications. Minimum 24 and 96 oligos required for 96- and 384-well plates, respectively. 200 pmol scale requires a minimum of 96 oligos.
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Duplexed Ultramer DNA Oligos
1 oligo pair per well, annealed. Shipped dry or resuspended in duplex buffer. Minimum of 24 and 96 oligos required for 96- and 384-well plates, respectively.
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The following annealing fee will be applied to each plate of duplexes:
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Product details
- Site-directed mutagenesis—used to efficiently and quickly generate large insertions, deletions or change stretches of sequence identity
- In vitro transcription—templates for the synthesis of RNA
- DNA-directed RNAi (ddRNAi)—hairpins are cloned into an expression vector, such as the GeneClip™ U1 Hairpin Cloning System (Promega)
- qPCR—as DNA standards
- CRISPR genome editing—as HDR templates
Every Ultramer DNA Oligo you receive will be deprotected and desalted to remove small molecule impurities. In addition, your oligos will be sequence-verified via proprietary ESI-mass spectrometry methods
* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.
Available modifications for Ultramer oligos
| Description | 200 pmol Ultramer DNA Oligo | 4 nmol Ultramer DNA Oligo | 20 nmol Ultramer DNA Oligo | PAGE Ultramer DNA Oligo |
|---|---|---|---|---|
| Availability | Plates | Plates and tubes | Plates and tubes | Tubes |
| 5' Phosphorylation | • | • | • | • |
| 5' Biotin | • | • | • | • |
| 5' Amino Modifier C6 | • | • | • | |
| 5' Amino Modifier C12 | • | • | • | |
| 5' deoxyInosine | • | • | • | • |
| 5' deoxyUridine | • | • | • | • |
| Int deoxyInosine | • | • | • | • |
| Int deoxyUridine | • | • | • | • |
| Phosphorothioate Bond | • | • | • | • |
| 2' O-Methyl RNA bases | • | • | • | • |
| 3' Amino Modifier | • | • | ||
| 5' 5-Methyl dC | • | • | • | |
| Int 5-Methyl dC | • | • | • | |
| 3' Phosphorylation | • | • | • | |
| Int Spacer 18 | • | • | • | |
| 3' C3 Spacer | • | • | • |
Quality
IDT proprietary DNA synthesis equipment permits rapid, high quality synthesis of nucleic acids. This platform is adjusted to a lower throughput mode which uses an "extra rich" synthesis cycle for long oligos. Along with this refined synthesis cycle, Ultramer DNA Oligos use a unique solid support that is specifically optimized to synthesize low-yield, high-quality oligos up to 200 bases in length. Overall, these improvements to our already established manufacturing methods allow you to acquire longer, purer oligos for your research. Figure 1 illustrates how we attain the exceptional quality of Ultramer DNA Oligos.
Figure 1. Full-length product received is determined by coupling efficiency. Ultramer DNA Oligos are manufactured on proprietary synthesis platforms that offer higher coupling efficiencies than industry standards. As seen in the graph, coupling efficiency becomes increasingly important as oligo length increases.
Our proprietary ESI-mass spectrometry methods allow us to confidently provide quality control documentation for your oligos up to 200 bases in length*. These QC traces, such as the examples shown in Figure 2, are available online free of charge.
* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.
Figure 2. QC data shows high purity of 185 nt and 189 nt Ultramer DNA Oligos. The measured molecular weight of each oligo is within 0.01% of its expected molecular weight. Data generated from in-house ESI-MS instruments.
Resources
Application guides
Brochures and flyers
Frequently asked questions
Tools
OligoAnalyzer™ tool
Identify oligonucleotide properties, including melting temperature, hairpins, dimers and mismatches
UNAFold tool
Perform secondary structure analysis
Dilution Calculator
Find the volume needed to dilute your oligo stock to a lower concentration
Resuspension Calculator
Find the volume needed to resuspend a dry oligo to a desired concentration
Services
Formulation
Diverse laboratory configurations and various individual preferences in the field require oligos that can be delivered in unique formats. With the formulation services listed below, you can have your oligos tailor-made to your specific requirements. Learn more »
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Custom quality control
In addition to the free ESI-mass spectrometry analysis that comes standard with your oligos*, your project may require one of the analytical services listed below. Learn more »
* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.
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GMP and OEM
If you require oligos that are approved for use in molecular diagnostic applications, or if you are interested in our third-party manufacturing services, please click here.
oPools™ Oligo Pools: Start with solid bases
We make them ready to use right out of the gate. Because why wouldn’t we?
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