Processing
CRISPR genome editing
HDR and Cas9 nickase design: What you should know
To achieve accurate and efficient genome editing with low off-target effects, approaches that result in homology-directed repair (HDR) are best. This requires using well designed gRNA pairs along with the Alt-R Cas9 nickases and carefully optimized experimental conditions.
- HDR and Cas9 nickase design: What you should know
- CRISPR-Cas9: What are the pros and cons?
- Primer design and other tools you should know about
- Single base editing
- Customizing CAR-T cells using the CRISPR-Cas9 system
- CRISPR knock-in: Tips and tricks
- CRISPRa and CRISPRi: Similarities and differences you should know about
- The CRISPR Basics Handbook: An IDT publication to help you learn CRISPR
- Genome editing tip: A CRISPR RNA annealing step can increase editing efficiency
- CRISPR GUARD: A short gRNA-based system that facilitates precise genome edits
- Alt-R™ HDR Donor Blocks can enable long DNA knock-ins while decreasing undesired edits
- Improving efficiency of homology-directed repair (HDR)
- CRISPECTOR, a statistical CRISPR genome editing analysis software for research
- A.s. Cas12a Ultra, a highly effective CRISPR genome editing enzyme
- A fusion gene identification strategy using CRISPR-Cas9 and long read nanopore sequencing
- Genome Oligopaint: Painting a clearer picture with oPools™ Oligo Pools
- Analyze your CRISPR genome edits with IDT's cloud-based software
- CRISPR-based depletion of unwanted sequences improves targeted next generation sequencing
- Applications and benefits of CRISPR functional genomics screens using arrayed guide RNA libraries
- SHERLOCK, PAC-MAN, and more: How CRISPR-Cas13 enzymes may be useful in the COVID-19 pandemic and beyond
- Stability of CRISPR reagents under different freezing, storage, and thawing conditions
- Consider ribonucleoprotein (RNP) instead of CRISPR plasmids!
- CRISPR fluorescent in situ hybridization in live cells: a new approach to monitoring chromosomal activities
- Prime editing with pegRNA: a novel and precise CRISPR genome editing system
- What is CRISPR screening?
- A mutant A.s. Cas12a (Cpf1) for CRISPR genome editing shows broad temperature tolerance and a high ratio of on:off target activity, with site-to-site reliability rivaling S.p. Cas9
- Similar editing efficiency with two-part and single guide RNA
- Using CRISPR-Cas9 technology for targeted nanopore sequencing
- High-fidelity Cas9 provides highly efficient genome editing with reduced off-target effects, even in primary cells
- When and how to use nickases for efficient genome editing
