gene_expression

rhAmp® SNP Genotyping System

Accurate, affordable genotyping with the next evolution of PCR

Improve the precision of your PCR-based SNP genotyping with rhAmp SNP Genotyping technology. This technology uses a unique two-enzyme system coupled with RNA-DNA hybrid primers to precisely interrogate target SNPs. Combined with IDT’s universal reporter chemistry, rhAmp SNP Genotyping offers a simple, high performance genotyping solution at an affordable price.

  • Generate the highest level of performance with greater than 99.5% call accuracy for over 90% of assays tested
  • Interrogate SNPs in difficult sequence regions with amplicon lengths as short as 40 bp
  • Validate markers affordably using the smallest pack size commercially available
  • Ensure confidence in your data with gBlocks® Gene Fragments as control templates

The rhAmp SNP portfolio includes all components needed to successfully generate high quality genotyping data on any commonly available real-time PCR instrument.

rhAmp SNP Assays

rhAmp SNP Assays offer a predesigned assay collection containing >10 million human SNPs, including a broad selection of functionally validated ADME SNP assays.

A custom assay design pipeline is also available for newly discovered human SNPs or assay designs of other species.

Ordering and product details »

rhAmp Genotyping Master Mix and Reporter Mixes

rhAmp Genotyping Master Mix and rhAmp Reporter Mixes are specially formulated for use with rhAmp SNP Assays to deliver superior specificity and signal generation.

Synthetic control templates are available during integrated ordering process.

Ordering and product details »

Fast and simple reaction setup

A single-tube assay setup allows for routine automation, and delivers genotypes with only 90 minutes of cycling time (Figure 1). Hot-start enzymes enable benchtop reaction setup, and stability of reactions for up to 3 days before and after cycling at room temperature.

Figure 1. Simple, one-tube reaction chemistry supports streamlined lab processes. All reagents are combined in the initial reaction setup that is stable for up to 48 hours at room temperature. Reaction setup, instrument run-time, and data acquisition may be completed in under 3 hours.

Superior chemistry for SNP detection

Superior discrimination versus traditional methods. Blocked primers minimize non-specific amplification. The 3' end of rhAmp primers incorporate a blocking group that prevents extension unless cleavage and de-blocking occur by RNase H2 enzyme. RNase H2 enzyme recognizes this RNA base only if it is hybridized to its perfect complement, initiating primer cleavage and activation.

Figure 2. Schematic representation of a rhAmp SNP Genotyping PCR cycle. All components needed to measure both alleles are combined in a single reaction before cycling. 1) Both allele-specific primers query the SNP locus. 2) RNase H2 enzyme cleaves the primers that are perfectly annealed to the target sequence, removing the RNA base and 3′ blocking modification, which allows extension by the IDT Taq Polymerase. 3) During the first two amplification cycles, a tail sequence is incorporated into the amplicon that is subsequently recognized by a universal, probe-based reporter system. 4) Polymerase extension leads to degradation of the probe and signal generation.