Polymerase chain reaction (PCR) is a core and widely used laboratory method. An enhancement of this method, qPCR (quantitative PCR, also known as real-time PCR) measures the amplification of DNA in real time and not at the end of cycling like conventional
PCR. Together these applications have contributed to significant advances in gene expression, genotyping, cloning, and other
applications. Central to PCR experimental success is the design and high-quality synthesis of primers or probes. Primers and probes that are prematurely truncated, contain incorrect base sequences, or include carryover contaminants from manufacturing
processes can impede accurate and efficient PCR.
Over several decades, we have refined a proprietary platform that improved nucleotide coupling efficiency during oligo synthesis. Subsequent
purification steps produce a high-quality primer or probe. All oligos are QC tested in-house by ESI mass spectroscopy* to help ensure an accurate, full-length, final product. Moreover, years of experience with nucleotide chemistry and thermodynamics
have been incorporated into advanced algorithms powering our easy-to-use sequence design tools.