oPools™ Oligo Pools

Start with solid bases

Use oPools Oligo Pools for accurate, reliable, and affordable CRISPR libraries; primer pools for multiplex PCR; gene construction; data storage; and FISH analysis. These pools of custom single-stranded DNA sequences offer high fidelity, uniformity, low error rates, and low dropout rates. This means that you can avoid amplification bias, varying concentrations, or high error rates that are often encountered when using pooled oligos from other suppliers.

Get started immediately: fast delivery and no amplification required
Reduce experimental variability with more complete coverage
Leverage your budget to screen more targets

Featured citation

Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome

Wegner M, Diehl V, et al..
eLife. doi: http://10.7554/eLife.42549 (2019)

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Ordering

oPools Oligo Pools

Pooled DNA oligos delivered dry in a single tube.

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Available in convenient sizes:

Scale (pmol/oligo)	Number of oligos per pool	Oligo length (bases)
1	100 to 20,000	40 to 350
10	10 to 2000	40 to 350
50	2 to 384	40 to 350

* Regardless of pool size, the first 3300 bases will be charged at the oPools DNA Base 1 rate. Bases 3301–50,000 will be charged at the oPools DNA Base 2 rate, bases 50,001–100,000 will be charged at the oPools DNA Base 3, and bases more than 100,000 will be charged at the oPools Base 4 rate.

The following service fees will be applied for modifications:

Modification

Price per oligo

5' Phosphorylation

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Product details

oPools Oligo Pools are pooled, high-fidelity single-stranded DNA sequences that are compatible with a variety of applications.

Proprietary DNA synthesis equipment permits rapid, high quality synthesis of nucleic acids. This platform is the same proprietary synthesis platform used to make IDT Ultramers. It uses an "extra rich" synthesis cycle for long oligos. Along with this refined synthesis cycle, oPools DNA oligos use a unique solid support specifically optimized to synthesize high-quality oligos up to 350 bases in length. Overall, these improvements to our already established manufacturing methods allow you to acquire longer, purer oligos for your research.

Specifications

Standard processing

Length

40–350 bases/oligo

Scale (number of oligos)

1 pmol/oligo	(100 to 20,000 oligos/pool)
10 pmol/oligo	(10 to 2000 oligos/pool)
50 pmol/oligo	(2 to 384 oligos/pool)

Mixed bases

N = A, C, G, and T
K = G and T
• Limited to 9 mixed bases per oligo

Modifications

5′ phosphorylation

QC and quantification

None

Shipping container

2 mL tube

Shipping and storage

Shipped dry
Turnaround time: 4–7 business days from order to delivery
Short-term storage, up to 6 weeks: room temperature (15 to 25°C) or 4°C
Long-term storage, up to 24 months: –20°C

Performance

Proprietary DNA synthesis equipment permits rapid, high quality synthesis of nucleic acids.

Figure 1 illustrates how our high coupling efficiency results in the exceptional quality of oPools oligo pools.

Figure 1. Amount of full-length product received is determined by coupling efficiency. oPool DNA oligos are manufactured using the same proprietary synthesis platform as Ultramer™ oligos (IDT) and offer higher coupling efficiencies than industry standards. As seen in the graph, coupling efficiency of oPools oligo pools (99.6%) allows for longer oligos to be synthesized in comparison to another supplier with a coupling efficiency of 98.5%.

Proprietary DNA synthesis equipment produces an even oligo yield distribution.

Figure 2 illustrates the yield uniformity of oPools oligo pools.

Figure 2. Proprietary DNA synthesis results in an even yield distribution of oPools oligos. This even yield distribution is shown here as a function of % difference from the mean. The standard deviation observed across ½ million sequences is less than 23% of the mean, demonstrating a high level of uniform sequence representation.

Proprietary DNA synthesis equipment results in exceptionally high sequence representation.

The high sequence representation is explained by very low dropout rates during synthesis. This is illustrated in Figure 3.

Figure 3. oPools oligo pools demonstrate exceptionally low dropout rates. Dropout rate refers to the likelihood that any individual sequence is not present in the final pool. This rate can vary by length and sequence complexity. The average dropout rate for oPools oligos across all length ranges was observed to be under 1% based on a sample size of over 900,000 oligos. Dropout rates are plotted by length range.