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Processing
oPools™ Oligo Pools
Start with solid bases
Use oPools Oligo Pools for accurate, reliable, and affordable CRISPR libraries; primer pools for multiplex PCR; gene construction; data storage; and FISH analysis. These pools of custom single-stranded DNA sequences offer high fidelity, uniformity, low error rates, and low dropout rates. This means that you can avoid amplification bias, varying concentrations, or high error rates that are often encountered when using pooled oligos from other suppliers.
oPools Oligo Pools
Pooled DNA oligos delivered dry in a single tube.
Available in convenient sizes:
| Scale (pmol/oligo) | Number of oligos per pool | Oligo length (bases) |
|---|---|---|
| 1 | 100 to 20,000 | 40 to 350 |
| 10 | 10 to 2000 | 40 to 350 |
| 50 | 2 to 384 | 40 to 350 |
* Regardless of pool size, the first 3300 bases will be charged at the oPools DNA Base 1 rate. Bases 3301–50,000 will be charged at the oPools DNA Base 2 rate, bases 50,001–100,000 will be charged at the oPools DNA Base 3, and bases more than 100,000 will be charged at the oPools Base 4 rate.
The following service fees will be applied for modifications:
Modification
Price per oligo
5' Phosphorylation
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Product details
oPools Oligo Pools are single-stranded DNA sequences that are used for CRISPR library construction, primer pools for multiplex PCR, gene construction, data storage, and FISH analysis.
Proprietary DNA synthesis equipment permits rapid, high-quality synthesis of nucleic acids. This platform is the same proprietary synthesis platform used to make IDT Ultramer™ DNA Oligos. It uses an "extra rich" synthesis cycle for long oligos. Along with this refined synthesis cycle, oPools DNA Oligos use a unique solid support specifically optimized to synthesize high-quality oligos up to 350 bases in length.
Specifications
| Standard processing | |||||||
|---|---|---|---|---|---|---|---|
| Length | 40–350 bases/oligo | ||||||
| Scale (number of oligos) |
| ||||||
| Mixed bases | N = A, C, G, and T K = G and T • Limited to 9 mixed bases per oligo | ||||||
| Modifications | 5′ phosphorylation | ||||||
| QC and quantification | None | ||||||
| Shipping container | 2 mL tube | ||||||
Shipping and storage
- Shipped dry
- Turnaround time: 4–7 business days from order to delivery
- Short-term storage, up to 6 weeks: room temperature (15 to 25°C) or 4°C
- Long-term storage, up to 24 months: –20°C
Product data
Proprietary DNA synthesis equipment permits rapid, high-quality synthesis of nucleic acids.
Figure 1. High coupling efficiency results in a greater amount of full-length product than other suppliers. oPools Oligo Pools are manufactured using the same proprietary synthesis platform as Ultramer™ DNA Oligos and offer higher coupling efficiencies than industry standards. The amount of full-length oligo is calculated using coupling efficiency. As seen in the graph, coupling efficiency of oPools Oligo Pools (99.6%) allows for longer oligos to be synthesized compared to coupling efficiency of 98.5% from three leading competitors.
Proprietary DNA synthesis equipment produces an even oligo yield distribution.
Figure 2. Proprietary DNA synthesis results in an even yield distribution of oPools Oligo Pools. This even yield distribution is shown here as a function of % difference from the mean. The standard deviation observed across ½ million sequences is less than 23% of the mean, demonstrating a high level of uniform sequence representation.
oPools Oligo Pools demonstrate low dropout rates
Dropout rate refers to the likelihood that any individual sequence is not present in the final pool. This rate can vary by length and sequence complexity. The average dropout rate for oPools oligos across all length ranges was 0.8% based on a sample size of over 900,000 oligos.
Resources
Frequently asked questions
How do I resuspend my oPools™ Oligo Pools to my desired concentration?
Calculate the appropriate resuspension volume for your oPools product with the IDT Resuspension Calculator.
First, convert the oPool scale—provided in pmol—to a nmol quantity.
If your desired final concentration is µM per oligo, use this equation to determine your quantity:
pmol per oligo / 1000 = # nmol per oligo
Example 1. Final concentration of 1 µM per oligo.
Scenario: 50 pmol oPools Oligo Pool
Quantity: 50 pmol oPools Oligo Pool / 1000 = 0.05 nmol
Final concentration: 1 µM
Resuspension calculator (Figure 1):
- Enter the quantity that you calculated
- Enter your final concentration
- Select Sgl-Stranded DNA (single-stranded DNA)
- Click Calculate
Figure 1. 50 pmol oPools Oligo Pool resuspended to 1 µM per oligo.
If your desired final concentration is nM per oligo, you can convert using this equation:
nM concentration / 1000 = # µM
Example 2. Final concentration of 100 nM per oligo.
Scenario: 50 pmol oPools Oligo Pool
Quantity: 50 pmol oPools Oligo Pool / 1000 = 0.05 nmol
Final concentration in µM: 100 nM / 1000 = 0.1 µM
Resuspension calculator (Figure 2):
- Enter the quantity that you calculated
- Enter your final concentration per oligo that you calculated
- Select Sgl-Stranded DNA (single-stranded DNA)
- Click Calculate
Figure 2. 50 pmol oPools Oligo Pool resuspended to 100 nM per oligo.
If your desired final concentration is per pool, use this equation:
(pmol per oligo x # of oligos in pool) / 1000 = # nmol per pool
Example 3. Final concentration of 1 µM per pool.
Scenario: 50 pmol oPools Oligo Pool containing 100 oligos
Quantity: (50 pmol x 100 oligos) / 1000 = 5 nmol
Final concentration: 1 µM
Resuspension calculator (Figure 3):
- Enter the quantity that you calculated
- Enter your final concentration
- Select Sgl-Stranded DNA (single-stranded DNA)
- Click Calculate
Figure 3. 50 pmol oPools Oligo Pool containing 100 oligos resuspended to 1 µM per pool.
Is each oligo that goes into oPools™ Oligo Pools manufactured individually?
Yes, all oligos that go into oPools Oligo Pools are synthesized separately. The oligos are pooled before shipment.
Are there any purification options available for oPools™ Oligo Pools?
oPools Oligo Pools go through the standard desalting process.
We do not offer additional purification as the manufacturing process is designed to be as efficient as possible.
If oPools™ Oligo Pools are not quantified, how are different yield options offered?
The individual oligos in oPools Oligo Pools are pooled based on an assumed average yield. This average yield was generated using historical data from previously synthesized IDT Ultramer™ oligos.
Are there any quality guarantees on oPools™ Oligo Pools similar to other IDT oligos?
As there are no QC checks performed on oPools Oligo Pools, there are no guarantees in terms of sequence identity or individual oligo purity.
While we expect that the vast majority of sequences ordered in the pool will be present, we cannot guarantee that any individual oligo is present in the pool.
Historical data has shown the percentage of oligos missing from the pool to be less than 1%.
What quality control is done on oPools™ Oligo Pools?
The oPools Oligo Pools manufacturing process is designed to be as efficient as possible.
Therefore, there are no quality control checks or quantification.
What diluent options are available for oPools™ Oligo Pools?
The oPools Oligo Pools manufacturing process is designed to be as efficient as possible. Therefore, we do not offer any formulation services on oPools Oligo Pools. All oPools Oligo Pools are delivered dry.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.
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