Please note that IDT Singapore will be closed on Thursday, 1st May Labor Day, Friday, 2nd May Polling Day, Monday, 12th May 2025 in observance of Vesak Day. For more information, please contact apac-custcare@idtdna.com.
The optimal Tm for most probes is 5-10°C higher than their associated primers. If your calculated Tm is too low, check out IDT's list of Tm enhancing mods, such as Affinity Plus.