PRIMERMIX Plates

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Plate Upload Instructions

Only one plate can be entered per tab
Empty wells do not need to be indicated
Download a sample Excel plate ordering template.

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1. Please specify an excel file.

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Product set options	Example
30-59 nt RNA including Alt-R™ modified RNA
60-150 nt RNA including Alt-R™ modified RNA
30-79 nt RNA including Phosphorothioated RNA/2'-O-methyl RNA
80-109 nt RNA including Phosphorothioated RNA/2'-O-methyl RNA
110-150 nt RNA including Phosphorothioated RNA/2'-O-methyl RNA

2. Review and select plates you would like to add

	Plate	# Wells

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Name

Sequence 1

Mod Code
	More Info
Mod Name Category

Mod Code
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Mod Name Category

Mod Code
	More Info
Mod Name Category

# Bases:

Name 2

Sequence 2

Mod Code
	More Info
Mod Name Category

Mod Code
	More Info
Mod Name Category

Mod Code
	More Info
Mod Name Category

# Bases:

Name

Sequence 1 Sequence Errors

Sequence 2

Sequence 1 Sequence Warnings

Sequence 2

Disclaimer for Clinical and Diagnostic Applications
IDT RUO products are manufactured in accordance with ISO 9001 and are intended for research use only. For clarity, RUO products are not intended for human use, including any clinical or diagnostic applications. If you are interested in using IDT products for clinical applications, please contact IDT application support to learn more about alternative manufacturing services available at IDT.

click here for ordering instructions.

Need an HDR donor design? Click here.

Plate(s) contains errors, please review plates and fix any errors before proceeding.

Plate(s) contains warnings, please review wells for warnings and click the ignore warnings checkbox to proceed.

Ignore Warnings

Convert Plate to RNA

Email Address

We will send final plate specifications to this email address.

Show CRISPR Help

Looking for controls?

NOTE:The same positive and negative controls can be used for both A.s. and L.b. Cas12a (Cpf1) nucleases.
To order, copy and paste the appropriate name and sequence from the following list into this ordering page:

Cas12a (Cpf1) positive control crRNAs

Item Name: Cas12a (Cpf1) Pos Ctrl crRNA, Human HPRT

Sequence: GGTTAAAGATGGTTAAATGAT

Item Name: Cas12a (Cpf1) Pos Ctrl crRNA, Mouse HPRT

Sequence: GGATGTTAAGAGTCCCTATCT

Item Name: Cas12a (Cpf1) Pos Ctrl crRNA, Rat HPRT

Sequence: ATGCTTAAGAGGTATTTGTTA

Cas12a (Cpf1) negative control crRNAs

Item Name: Cas12a (Cpf1) Neg Ctrl crRNA #1

Sequence: CGTTAATCGCGTATAATACGG

Item Name: Cas12a (Cpf1) Neg Ctrl crRNA #2

Sequence: CATATTGCGCGTATAGTCGCG

Item Name: Cas12a (Cpf1) Neg Ctrl crRNA #3

Sequence: GGCGCGTATAGTCGCGCGTAT

Help

Cas12a (Cpf1) positive control crRNAs
Item Name:	Cas12a (Cpf1) Pos Ctrl crRNA, Human HPRT
Sequence:	GGTTAAAGATGGTTAAATGAT
Item Name:	Cas12a (Cpf1) Pos Ctrl crRNA, Mouse HPRT
Sequence:	GGATGTTAAGAGTCCCTATCT
Item Name:	Cas12a (Cpf1) Pos Ctrl crRNA, Rat HPRT
Sequence:	ATGCTTAAGAGGTATTTGTTA

Cas12a (Cpf1) negative control crRNAs
Item Name:	Cas12a (Cpf1) Neg Ctrl crRNA #1
Sequence:	CGTTAATCGCGTATAATACGG
Item Name:	Cas12a (Cpf1) Neg Ctrl crRNA #2
Sequence:	CATATTGCGCGTATAGTCGCG
Item Name:	Cas12a (Cpf1) Neg Ctrl crRNA #3
Sequence:	GGCGCGTATAGTCGCGCGTAT

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Base Notations

Standard Bases, (A, C, G, T) are not case sensitive.
Mixed Bases, expressed in UPPERCASE.
RNA, expressed as 'r_', for example rA, rU.
Base notation for Affinity Plus (locked nucleic acids) = +A, +G, +C, +T
2' O-methyl RNA bases are entered as 'm_'.
Phosphorothioated DNA bases are entered as '_*'
Phosphorothioated RNA bases are entered as 'r_*'.
Phosphorothioated 2'-O-methyl RNA bases are entered as 'm_*'.

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Base Notations