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We have observed that dose response curves vary for different guide sequences, depending on potency of the guide. We recommend titrating the amount of RNP and keeping the amount of enhancer fixed.
For the Nucleofector® System (Lonza), we recommend 3 µM of Alt-R Cpf1 Electroporation Enhancer, and for the Neon® System (Thermo Fisher Scientific), we recommend 1.8 µM of Alt-R Cpf1 Electroporation Enhancer. Toxicity may be observed at high concentrations of enhancer.