NGS Discovery Pools are high-fidelity, individually-synthesized, 5′-biotinylated oligos for targeted NGS. Proprietary DNA synthesis equipment permits rapid, high-quality synthesis of nucleic acids. These nucleic acid probes can be used for creating custom capture panels that can be optimized, expanded, and combined with other panels. Improvements to our established manufacturing process allow you to build custom panels quickly at a fraction of the cost of conventional custom panels.
NGS Discovery Pools are pooled, high-fidelity, single-stranded DNA probes for targeted next generation sequencing. Create custom capture panels that can be optimized, expanded, and combined with other panels. Panels ship in as few as 5 business days (<50,000 probes).
Proprietary DNA synthesis equipment permits rapid, high-quality synthesis of nucleic acids. This platform is the same proprietary synthesis platform used to make IDT Ultramers. It uses an "extra rich" synthesis cycle for long oligos. Along with this refined synthesis cycle, these improvements to our already established manufacturing methods allow you to acquire longer, purer oligos for your research.
We can help you optimize your panel with NGS functional testing.
Shipping and storage recommendations:
NGS Discovery Pools produce consistent coverage for a range of multiplexed captures (Figure 1).
NGS Discovery Pools were tested with libraries created from human genomic DNA (Coriell). 500 ng library per sample was used to capture using the standard IDT protocol. The enriched libraries were sequenced on a NextSeq® instrument (Illumina) as indicated and standard Picard Metrics calculated. Data shown is the average for an 8-plex capture (Table 1).
Table 1. NGS Discovery Pools custom panel performance.
|Name||Target size (kb)||Probe count||Genes targeted||Reads per sample (150 bp read length)||% selected bases||Mean target coverage||% target at 30+ X||Duplicate rate %|