Use of NCBI databases in qPCR assay design

In this video, Dr Elisabeth Wagner, Scientific Applications Specialist at IDT, covers: 1. Which NCBI tools are useful for designing qPCR assays 2. Using tools for qPCR design in the IDT SciTools® suite 3. Features and tools available on the NCBI website 4. Obtaining sequence information for your gene of interest 5. Performing a BLAST search for assay specificity 6. Searching for SNPs 7. Understanding how to proceed with a basic qPCR design This presentation was given on April 15th, 2014 at the qPCR Workshop at the University of Iowa BioVentures Center.

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