qPCR design strategies for specific applications
In this video, Dr Elisabeth Wagner, Scientific Applications Specialist at IDT, covers:
1. Understanding the different types of design specifications for species and splice-form specific qPCR and CNV assays
2. Identifying design considerations for different experimental scenarios and adjusting the basic qPCR design parameters accordingly
3. How to do an alignment of sequences to discover both unique and similar regions
4. How to design Copy Number Variations assays
This presentation was given on April 15th, 2014 at the qPCR Workshop at the University of Iowa BioVentures Center.