Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Should I amplify my gBlocks™ HiFi Gene Fragments when I receive them?

Most applications such as restriction-digest, blunt-end, and Gibson cloning can be performed with the material provided by IDT, and PCR amplification should not be necessary. If the purpose of the amplification is to add flanking bases to complete a cloning reaction, we strongly recommend ordering the gBlocks™ HiFi Gene Fragment (IDT) with the required bases rather than adding them afterwards using PCR.

If you must amplify your gBlocks HiFi Gene Fragment, use the minimum amount of starting template required by your PCR kit protocol, limit the cycle number to 10–12, and use a high-fidelity polymerase.



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