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Is titration of adapters required for different input amounts with the xGen™ DNA Library Prep Kit EZ / MC?

xGen DNA Library Prep Kit EZ

For direct sequencing of libraries prepared using the xGen DNA Library Prep Kit EZ from DNA inputs <25 ng, adapter dilution is necessary to achieve low levels of adapter dimers.

DNA input (ng)Reagent W4
≥25No dilution
 10 10-fold (1:10)
 1 20-fold (1:20)

xGen DNA Library Prep Kit MC

For direct sequencing of libraries prepared using the xGen DNA Library Prep Kit MC from DNA inputs <50 ng, adapter dilution is necessary to achieve low levels of adapter dimers. Adapter titration recommendations are outlined in the table below. Optimization may be required for diluting adapters of intermediate input scale.
 
DNA input (ng)Adapter
≥50No dilution
 10 10-fold (1:10)
 1 20-fold (1:20)

Categories:
Next generation sequencing

Tags:
ngs adapter Next generation sequencing