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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Is library amplification required when using the xGen DNA Library Prep Kits?
Library amplification is required if Tru-Seq-compatible indexing primers are used in the library preparation. However, if library amplification is not performed (i.e., when doing a PCR-free workflow with ≥ 100 ng of library input), Tru-Seq-compatible full-length Y adapters must be used for adapter ligation. When Tru-Seq-compatible full-length Y adapters are used in the library prep, library amplification may or may not be required, depending on the amount of library needed for your downstream application.
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Next generation sequencing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.