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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How much xGen Stubby Adapter and Unique Dual Index Primer Pairs should I use for library prep?

The amount of xGen Stubby Adapter and Unique Dual Index Primer Pairs you use depends on the library prep workflow and the amount of starting sample used to perform library prep. We recommend checking your specific protocol for the optimal amount of adapter and indexing primers to use.

For the Lotus DNA Library Prep kit, use 4 µL of xGen Stubby Adapter (diluted according to the table below) for the ligation step. For the library amplification step, use 5 µL of xGen Unique Dual Index Primer Pairs.

DNA input (ng) Adapter dilution Stock concentration (µM)
≥25 No dilution 15
10 10-fold (1:10) 1.5
1 20-fold (1:20) 0.75

If diluting the xGen Stubby Adapter is required, we recommend using Nuclease-Free Duplex Buffer. Nuclease-Free Duplex Buffer contains salts, which help keep the adapter duplexed.

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.