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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
How do I sequence the UMI in xGen™ UDI-UMI Adapters?
The UMI (dark blue in the illustration below) is positioned next to the i7 index (light orange) so that it will be sequenced as part of the i7 index read.
So, to sequence the UMI, increase the number of cycles for the i7 index read by 9 cycles. Refer to the IDT Master Index List for guidelines concerning the xGen™ UDI-UMI adapters.
Categories:
Next generation sequencing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.