The UMI (dark blue in graphic) is positioned next to the i7 index (light orange) so that it will be sequenced after the i7 index. Therefore, to sequence the UMI, increase the number of cycles for the i7 index read by 9 cycles.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.