Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How do I design my gBlocks™ HiFi Gene Fragment for restriction cloning?

Cloning of gBlocks HiFi Gene Fragments (IDT) is similar to cloning a very pure PCR product. For restriction cloning, it is important to add 6–8 nt at the ends of the fragment, beyond the restriction recognition sequence. Most restriction enzymes will not cut efficiently, or at all, if the fragment terminates precisely at the restriction site.



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