Do I need library quantification after amplification and before Normalase™ I enzyme?
No, it is not necessary to quantify libraries before Normalase™ I enzyme if the libraries are all known to meet the 6 nM or 12 nM yield threshold. For example, if your library preparation and your standard nucleic acid sample inputs consistently yield 20 nM, quantification can be skipped.
Quantification is only required when library yields are unknown, and potentially when they are under the 6 nM or 12 nM minimum threshold.
For quality control purposes, we recommend quantifying libraries after Normalase PCR using a validated qPCR assay or fluorometric quantification assay.