We do not recommend performing library amplification directly in the 96-well plate used to deliver the xGen Unique Dual Index Primer Pairs.
While the amount of indexing primers in each well is enough for 1 reaction, extra indexing primer is provided to ensure there is sufficient material. Including the excess primers will increase the risk of forming primer dimers during PCR.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.