CRISPR GUARD

CRISPR GUARD

Precise genome editing using CRISPR-Cas9 is a promising avenue for genetic disease research. However, there are a number of off-target editing issues to take into consideration.

In this webinar, Dr. Matthew Coelho discusses findings from his recent publication in Nature Communications, "CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs."

Attendees will learn more about:

  1. The utility of CRISPR Guide RNA Assisted Reduction of Damage (GUARD) as a method for protecting off-targets sites by co-delivery of short guide RNAs directed against off-target loci by competition with the on-target guide RNA.
  2. Project design rules and the versatility of this method for in vitro studies and high-throughput screening.

Published on: August 03, 2022