Increasing genome editing efficiency and specificity with optimized CRISPR-Cas9 guide RNAs
Use of CRISPR-Cas9 has revolutionized targeted genome editing. The process requires the Cas9 enzyme and guide RNA (gRNA), which directs Cas9 to the target DNA location. The gRNA can be generated using a variety of methods. In this webinar, Ashley Jacobi compares the on-target and off-target editing events observed with different gRNA formats in transformed and primary cell lines. She provides guidelines on when to use each gRNA in the context of your Cas9 source (plasmid, mRNA, ribonucleoprotein complex) and cell type.