How to optimize non-viral CRISPR HDR for high-efficiency large knock-in in primary T cells and iPSCs

How to optimize non-viral CRISPR HDR for high-efficiency large knock-in in primary T cells and iPSCs

Achieving large knock-ins, such as chimeric antigen receptor (CAR) insertions in primary T lymphocytes, remains a key challenge in genome editing. In this webinar, we present our findings from extensive bench research focused on optimizing CRISPR homology-directed repair (HDR) techniques in CAR knock-ins in primary T cells. We cover crucial factors including double strand break repair mechanisms, CRISPR reagent selection, and optimized cell handling workflows. Additionally, we highlight the use of Aldevron Nanoplasmids™ as highly efficient HDR donor templates, successfully demonstrated in both primary T cells and induced pluripotent stem cells (iPSCs). The session concludes with a workflow summary and a Q&A session.

Published on: November 20, 2024