Don’t Worry, Bead Happy: Essential Knowledge for Microparticle Use in DNA and RNA Analysis

Many protocols in genomic analysis use microparticles as a mobile solid phase. Their utility extends to DNA and RNA isolation, hybridization-based capture, SNP detection, isolation of nucleic acid binding proteins, and target enrichment for NGS. The first portion of the presentation will describe these approaches and delve into the attachment chemistry and oligonucleotide design considerations for microparticle-based approaches. The discussion will also examine particle attributes, characterization methods, and best practices for developing particle reagents and processes.