26 Citations found

Blazejewski T, Ho HI, Wang HH. (2019) Synthetic sequence entanglement augments stability and containment of genetic information in cells. Science. doi: 10.1126/science.aav5477

Constraining Adaptive Mutations using Engineered Overlapping Sequences (CAMEOS) is a new computational platform that uses overlapping sequences to prevent protein function disruption. Read how Blazejewski, et al, used gBlocks Gene Fragments and tested their algorithm to ensure that synthetic protein encoding is safeguarded against mutations.

The authors used gBlocks® Gene Fragments to generate a standard curve for their qPCR analysis.

The authors used gBlocks® Gene Fragments to generate a standard curve for their qPCR analysis.

Perry AS, Baird AM, Gray SG. (2015) Epigenetic Methodologies for the study of Celiac Disease. Methods Mol Biol, 1326 : 131–158.

The authors used gBlocks® Gene Fragments to generate a standard curve for their qPCR analysis.

Feng X, Lian J, Zhao H. (2015) Metabolic engineering of Saccharomyces cerevisiae to improve 1-hexadecanol production. Metab Eng, 27 : 10–19.

gBlocks Gene Fragments were used to generate the fatty acyl-CoA reductase gene from barn owl.

Gunawardana M, Chang S, et al. . (2014) Isolation of PCR quality microbial community DNA from heavily contaminated environments.. J Microbiol Methods, 102 : 1–7.

The authors used gBlocks® Gene Fragments to generate a standard curve for their qPCR analysis.

The authors used gBlocks® Gene Fragments to generate a standard curve for their qPCR analysis.

Liachko I, Youngblood RA, et al. (2014) GC-rich DNA elements enable replication origin activity in the methylotrophic yeast Pichia pastoris. PLoS Genet, 10 : e1004169.

gBlocks Gene Fragments were used to generate mutant autonomously replicating sequences, or ARSs, associated with replication origins in yeast chromosomal DNA.

Ghorbal M, Gorman M, et al. (2014) Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system. Nat Biotechnol, 32 : 819–821.

A gBlocks Gene Fragment was used to create an sgRNA expression vector for use with the CRISPR/Cas9 system.

Briner AE, Donohoue PD, et al.. (2014) Guide RNA functional modules direct Cas9 activity and orthogonality. Mol Cell, 56 : 333–339.

gBlocks Gene Fragments were used to create sgRNA expression plasmids. 

Marbiah MM, Harvey A, et al. (2014) Identification of a gene regulatory network associated with prion replication. EMBO J, 33 : 1527–1547.

5 gBlocks Gene Fragments were used to assemble the ~2 kb coding sequence for Micalcl (NM_027587).

Heel T, McIntosh JA, et al. (2014) Non-natural olefin cyclopropanation catalyzed by diverse cytochrome P450s and other hemoproteins. Chembiochem, 15 : 2556–2562.

gBlocks Gene Fragments were used to generate codon-optimized cytochrome P450 enzymes, and other hemoproteins, in order to study their catalytic use in non-natural olefin cyclopropanation reactions.

Port F, Chen HM, et al. (2014) Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci USA, 111 : E2967–2976.

gBlocks Gene Fragments were used to construct a codon optimized Cas9 sequence for expression in Drosophila. Optimization of the sequence was performed using the IDT Codon Optimization tool.

O'Connell MR, Oakes BL, et al.. (2014) Programmable RNA recognition and cleavage by CRISPR/Cas9. Nature, 516 : 263–266.

gBlocks Gene Fragments were used to create sgRNA, gene specific sequences.

Lindqvist LM, Heinlein M, et al. (2014) Prosurvival Bcl-2 family members affect autophagy only indirectly, by inhibiting Bax and Bak. Proc Natl Acad Sci USA, 111 : 8512–8517.

gBlocks Gene Fragments were used to generate codon-optimized acRAF genes from <em>H. neapolitanus</em> and <em>P. marinus</em>.

Cervinski MA, Sam SS, et al. (2014) Validation of interleukin 28B genotyping assay for clinical use. Clin Biochem, 47 : 478–480.

gBlocks Gene Fragments were used as positive genotype controls for the described 5′ nuclease, SNP genotyping assay. 

gBlocks Gene Fragments were used for creating sgRNA expression constructs for targeting mutant Cas9 variants to test either transcription activation or genome modification.

Gilbert LA, Larson MH, et al. (2013) CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell, 154 : 442–451.

gBlocks Gene Fragments were used to create an Mxi1 transcription repressor domain and assembled with a dCas9 construct using the Gibson Assembly™ method into an expression vector. The resulting chimeric, transcriptional repressor was then shown to be targetable using the CRISPR/Cas9 mechanism.

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