{"id":905,"date":"2016-05-09T05:00:00","date_gmt":"2016-05-09T05:00:00","guid":{"rendered":"https:\/\/www.idtdna.com\/page\/n6-methyladenosine-m6a-modulating-rna-localization-structure-stability-splicing-and-translation"},"modified":"2025-08-22T16:10:32","modified_gmt":"2025-08-22T16:10:32","slug":"n6-methyladenosine-m6a-modulating-rna-localization-structure-stability-splicing-and-translation","status":"publish","type":"post","link":"https:\/\/sg.idtdna.com\/page\/support-and-education\/decoded-plus\/n6-methyladenosine-m6a-modulating-rna-localization-structure-stability-splicing-and-translation\/","title":{"rendered":"N6-methyladenosine (m6A)\u2014Modulating RNA localization, structure, stability, splicing, and translation"},"content":{"rendered":"<h3>Quick facts about N6-methyladenosine (m6A)<\/h3>\n<p>Availability: RNA<strong> Location:<\/strong> 5&prime;, internal, and 3&prime;<strong> Scales:<\/strong> 250 nmol to large scale<strong> Purification:<\/strong> Standard desalt or HPLC<strong> Ordering:<\/strong> \/iN6Me-rA\/<\/p>\n<h3>A popular off-catalog oligo modification<\/h3>\n<p>We welcome requests for oligo modifications that do not appear in our <a href=\"\/pages\/products\/custom-dna-rna\/oligo-modifications\">website listing<\/a>. These are regularly reviewed for approval by our specialty oligo synthesis committee, with some made available for quoting and ordering online directly. One popular modification is N6-methyladenosine (m6A). This is a modified RNA base with a methyl group at the N6 position (Figure 1).<\/p>\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/www.idtdna.com\/page\/wp-content\/uploads\/idt-images\/798b5d15-3279-6e2e-aa53-ff00001c1b3c-art131-mh-n6-me-ra-fig1.png\" title=\"D-OLI16PS-n6-F1\" data-displaymode=\"Original\" alt=\"art131-MH-N6-Me-rA-Fig1\" \/><figcaption class=\"image-caption\">Figure 1. Structure of N6-methyladenosine.<\/figcaption><\/figure>\n<p>First described in the 1970s, m6A is a prevalent internal (non-cap) mRNA modification found in many eukaryotes [<a href=\"#references\" data-sf-ec-immutable=\"\">1<\/a>]. It also occurs in other RNA species (tRNA, rRNA, snoRNA, lncRNA) and in a wide variety of organisms, including insects, plants, and yeast [<a href=\"#references\" data-sf-ec-immutable=\"\">2&ndash;5<\/a>]. It is essential for cell viability and development [<a href=\"#references\" data-sf-ec-immutable=\"\">4&ndash;6<\/a>], and has been associated with mRNA localization, structure, stability, splicing, and translation [<a href=\"#references\" data-sf-ec-immutable=\"\">7<\/a>]. <\/p>\n<p>In humans, this methylation event is a natural post-transcriptional modification carried out by a complex consisting of METTL3 and METTL14 methyltransferases and uses the consensus sequence [G\/A\/U][G&gt;A]m6AC[U&gt;A\/C] [<a href=\"#references\" data-sf-ec-immutable=\"\">2,3<\/a>]. Human and mouse transcriptome-wide analysis studies and mapping by m6A-CLIP\/IP have revealed that the majority of m6A modifications are associated with regions of high evolutionary conservation and are&nbsp;preferentially located in the most 3' exon, suggesting the potential for 3'UTR regulation, including alternative polyadenylation [<a href=\"#references\" data-sf-ec-immutable=\"\">2,8<\/a>].<\/p>\n<p>Demethylation of m6A modified bases also occurs naturally within cells. The fat mass and obesity associated (<em>FTO<\/em>)<em> <\/em>gene encodes an enzyme that removes the methyl group from this modified base, and overexpression of <em>FTO <\/em>results in a lower level of m6A in mRNA [<a href=\"#references\" data-sf-ec-immutable=\"\">2<\/a>]. The AlkB homolog 5, RNA demethylase (<em>ALKBH5<\/em>) gene encodes another RNA demethylase responsible for specific demethylation of m6A residues.<\/p>\n<h3>How to order m6A modifications<\/h3>\n<p>To order RNA oligonucleotides containing m6A modifications, use \/iN6Me-rA\/ to specify this nucleotide in the sequence you provide. Oligos containing this modification can be ordered with <a href=\"\/pages\/products\/optional-services\/page-hplc-purification\">HPLC purification<\/a>, or with standard desalt, provided that other modifications in the sequence do not require HPLC.<\/p>\n<h3>Learn about other oligo modifications to facilitate your research<\/h3>\n<p>See what other modifications are available from IDT. You can find a list of standard modifications we make to oligonucleotides on the&nbsp;<a href=\"\/pages\/products\/custom-dna-rna\/oligo-modifications\">Modifications page<\/a>&nbsp;of our online catalog. And if you don&rsquo;t find what you are looking for, just send us a request. We often synthesize special, non-catalog requests. <a href=\"\/pages\/about\/contact-us\">Contact us<\/a> to learn more.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Quick facts about N6-methyladenosine (m6A) Availability: RNA Location: 5&prime;, internal, and 3&prime; Scales: 250 nmol to large scale Purification: Standard desalt or HPLC Ordering: \/iN6Me-rA\/ A popular off-catalog oligo modification We welcome requests for oligo modifications that do not appear in our website listing. These are regularly reviewed for approval by our specialty oligo synthesis [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"ct_builder_shortcodes":"","ct_template_type":"","ct_parent_template":0,"inline_featured_image":false,"footnotes":""},"class_list":["post-905","post","type-post","status-publish","format-standard","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>N6-methyladenosine (m6A) Modification | IDT<\/title>\n<meta name=\"description\" content=\"Read about the mechanism of methylation and functions of N6-methyladenosine (m6A) modifications in the cell.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" 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