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  3. Master mixes & reagents
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qPCR and PCR master mixes, enzymes and reagents

Using commercially produced master mixes, enzymes, and reagents ensures simple, efficient experiment setup and consistent data.

PrimeTime™ Gene Expression Master Mix

PrimeTime Gene Expression Master Mix is a hot-start master mix delivering high quality results under either conventional or fast cycling conditions. Optimized for probe-based qPCR.

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PrimeTime™ One-Step RT-qPCR Master Mix

The PrimeTime One-Step RT-qPCR Master Mix is a ready-to-use, 2X concentrated mixture that is designed for use in probe-based, real-time quantitative PCR. It contains an antibody-mediated hot-start DNA polymerase, reverse transcriptase, dNTPs, MgCl2, enhancers, and stabilizers. PrimeTime One-Step RT-qPCR Master Mix offers high quality results for routine one-step qPCR experiments.

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PrimeTime™ One-Step 4X Broad-Range Master Mix

PrimeTime One-Step 4X Broad-Range Master Mix is a ready-to-use 4X concentrated mixture that is designed for use in probe-based, real-time quantitative PCR. It is a premium one-step qPCR master mix that offers advanced capabilities including the option to amplify from crude or purified samples. It contains a hot-start reverse transcriptase, hot-start DNA polymerase, dNTPs, buffer components, and an enhancer solution which improves direct amplification from difficult samples.

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rhAmp™ Genotyping Master Mix and Reporter Mixes

rhAmp Genotyping Master Mix and rhAmp Reporter mixes are specially formulated for use with rhAmp SNP Assays to deliver enhanced allelic discrimination and signal generation. Synthetic control templates are available during the integrated ordering process.

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RNase H2 enzyme

Increase the precision of target sequence amplification by limiting negative effects from primer-dimers. For use in customer-developed rhPCR assay applications, such as rare variant identification, splice variant discovery, or microbial identification studies.

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Nuclease detection and control

Reagents for rapid detection and elimination of RNases and DNases. The nuclease detection kits, effective for testing lab reagents, equipment, and other lab supplies, can be read visually for qualitative assessment of contamination or quantified using fluorometry.

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Overview

Using commercially produced master mixes, enzymes, and reagents ensures simple, efficient experiment setup and consistent data. Each batch of reagents from IDT undergoes rigorous quality assessments for performance and reproducibility, moving you one step closer to achieving successful and consistent qPCR results.

Mastermix2-step RT-qPCR1-step RT-qPCRDirect amplification from crude samples2X4XInhibitor resistantShipping condition
PrimeTime Gene Expression Master Mix✔  ✔ ✔Ambient temperature
PrimeTime One-Step RT-qPCR Master Mix ✔ ✔ ✔Dry ice
PrimeTime One-Step 4X Broad-Range Master Mix ✔✔ ✔✔✔Dry ice
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